HT400E Automated SPE System
and Sample Injector

Product Information

• Automated sequential SPE system
  
  
• Built-in HPLC injector
  
  
• Process up to 20 cartridges
  
  
• 1ml and 3ml SPE cartridges
  
  
• Unlimited sample processing steps
  Competitively priced
  
  
• Based on existing proven technology

The new HT400E is a fully automatic Solid Phase Extraction system and HPLC autosampler for unattended sample preparation and HPLC analysis.

The system is able to sequentially process up to 20 SPE tubes of 1ml or 3ml capacity. It offers multiple options for cartridge conditioning, sample loading, washing, drying, elution, dilution, derivatization and injection.

Different syringe size options are available to handle various amounts of sample and reagents.

Based on the existing and extremely successful range of autosampler robots from Hta, the hardware is already well tested for reliability and ease of support.

A standard HT400E system is configured for five reagents with one wash solution, one waste station and a sample injector. The modular design of the system enables multiple options for the number of reagents and wash solutions.

For each sample, the tray contains one SPE cartridge position, a sample position, an elution position and one position for an extra step such as dilution or derivatization.

The control software allows easy set-up of the sample processing sequence and multiple methods can be stored and modified at any time. The flexibility of the software allows almost any methodology to be created.

Special plungers are placed in the SPE tubes that allow the syringe needle to directly and accurately deliver solution to the surface of the solid phase in the tube. The plungers also allow the unit to physically lift and move the SPE cartridge to the wash, waste and elution stations as required.

The final sample is then injected via a built-in HPLC injector connected directly to the HPLC system. The run start/stop is controlled via contact closures with the HPLC system.

Applications:
There are many applications for automated SPE. A number of which have already been run on the system including:

• Vanillylmandelic Acid Method 1 & Method 2
• Metanephrine Method 1 & Method 2
• Benzodiazapine
• Tricyclic Antidepressant drugs
• Pyridinoline and Deoxypyridinoline

Many more applications are in preparation, including OPA derivatization of Amino Acids. These will be added over the coming months.

Specifications

Additional Information

HT400E Brochure (677KB)